Laboratory Professionals

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Proper collection, transportation, and handling of urine specimens are important to avoid contamination, or deterioration of constituents. This is where the complete KOVA® Urinalysis System differentiates itself from conventional urinalysis products. It is designed to eliminate significant sources of variation, assuring the right precision and accuracy, test after test. It includes everything the lab needs to standardize critical steps from sample collection to centrifugation, decanting and resuspension of sediment, slide preparation and reporting results.

What Makes the KOVA® System Components Unique?

- Secure Fit

The secure fit of the KOVA® Tubes and Caps prevent spillage during specimen collection, transportation, and handling, minimizing biohazardous exposure as well as reducing the risk of aerosol contamination during centrifugation.

- Unique Petters

The unique KOVA® Petters assure volume consistent sediment collection and suspension with one-step, contamination-free decanting.

- Precision Manufacturing

The precision manufactured KOVA® Glasstic 10 Slides feature specially designed wells for precise volume control, as well counting grids, eliminating variables that commonly interfere with microscopic analysis. Other slide products can only hold one or two samples, have seams between wells that increase the risk of sample cross-contamination, tend to break more easily, or disperse smaller particles to the edges due to adhesion forces, resulting in miscounts.

- Lab Standards

The KOVA® system conforms to CLIA (Clinical Laboratory Improvement Amendments) and (CLSI) Clinical and Laboratory Standards Institute Guidelines.


How to Use the KOVA® System

The KOVA® System is so easy that an untrained laboratory technician with less than 30 minutes of instruction can obtain the same results as an experienced technologist. It includes specially engineered microscopic slides, sample tubes & caps, custom pipettes or “petters”, collection cups and multi-level urinalysis controls for reliable, simple and efficient urinalysis testing and standardization.

Centrifuging
  • Fill the KOVA® Tube to 12mL and firmly attach the KOVA® Cap
  • Centrifuge at 400 rcf (1500 rpm) for 5 minutes



  • Decanting
  • Insert the KOVA® Petter firmly and decant
  • 1.0mL of sediment will be trapped by the KOVA® Petter



  • Resuspension
  • Gently resuspend using the KOVA® Petter
  • If desired, add 1 drop of KOVA® Stain prior to resuspending



  • Slide Preparation
  • Using the KOVA® Petter, transfer the sample to the cut-out notch on the slide chamber
  • Careful addition of samples insures the hygienic handling properties of the KOVA® System



  • Sediment Suspension
  • 6.6 µL of the sample will be drawn into the KOVA® Slide 10 chamber via capillary action resulting in a homogenous suspension of the sediment



  • Count
  • Quantitate the casts at low power (100x)
  • Quantitate all cells at high power (400x)
  • Count the cells within the lines of the small 0.33 mm square grid (as shown)
  • Refer to the value table for the cell count per µL of patient sample

  • Counting Cells with the KOVA® Glasstic 10 Slides

    To download the cell count value tables click here:


    For Clinicians

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    The KOVA® Urinalysis System includes everything the lab needs to standardize critical steps from sample collection to centrifugation, decanting and resuspension of sediment, slide preparation, and quality control. Why is this relevant to you?

    Between 32% and 75% of all testing errors in urinalysis occur in the preanalytical phase*. Proper collection, transportation, and handling of urine specimens are important to avoid contamination, or deterioration of constituents. The secure fit of the KOVA® Tubes and Caps prevent spillage during specimen collection, transportation, and handling, minimizing biohazardous exposure as well as reducing the risk of aerosol contamination during centrifugation.

    The unique KOVA® Petters assure volume consistent sediment collection and suspension with one-step, contamination-free decanting.

    The precision manufactured KOVA® Glasstic Slides feature specially designed wells for precise volume control, as well counting grids, eliminating variables that commonly interfere with microscopic analysis. slide-grid-detail-pop-out KOVA® Stain is a modified Sternheimer-Malbin stain that provides higher contrast for rapid and accurate identification of urinary sediment particles.

    To assure the proper daily QC of macroscopic, microscopic, and chemical analysis of your patient’s urine specimens KOVA® offers KOVA-Trol® and KOVA Liqua-Trol®, urine-based controls that simulate both normal and abnormal levels of key analytes, as well as microscopics for sediment analysis.

    The KOVA® Urinalysis System conforms to CLIA (Clinical Laboratory Improvement Amendments) and (CLSI) Clinical and Laboratory Standards Institute Guidelines.

    This is where the complete KOVA® Urinalysis System differentiates itself from conventional urinalysis products. Ask if your laboratory uses the KOVA® Urinalysis System to assure better results.


    For Patients

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    Analysis of urine specimens is the most commonly performed laboratory test in medicine today. Urine can be evaluated for:

  • Physical Properties – Cloudy, red, or reddish-brown may mean that you have blood, pus, or kidney stones in your urine. Particular odors such as a maple syrup, rotting fish, or a mousy smell can indicate certain diseases.
  • Chemical Properties – Reagent strips (specially treated strips of paper) or dipsticks can provide information on the acidity of your urine and whether it contains blood, protein, or sugar.
  • Microscopic Properties – Can show bacteria, red or white blood cells, and tumor cells in the urine when evaluated under a microscope.
  • Urinalysis is used to screen for kidney infections, stones, or disease, diabetes mellitus, metabolic diseases, liver problems, hypertension, liver disease, urinary tract infection, lupus, congestive heart failure, cancer, muscle breakdown, blood in the urine, and various other related conditions.

    KOVA® International developed the KOVA® Urinalysis System, which consists of specially designed tubes, caps, decanters, slides, stains and QC products to standardize the critical steps from sample collection to analysis in the lab. The KOVA® Urinalysis System reduces significant sources of error, assuring that the doctor gets more dependable results to support your diagnosis and treatment. Ask if your laboratory uses the KOVA® Urinalysis System to assure better results.

    For more information about urinalysis:


    Resources

    Publications, journal articles and materials regarding urinalysis.

    • Urinalysis; Approved Guideline – Third Edition. CLSI Document GP16-A3. (ISBN 1-56238-687-5). Clinical and Laboratory Standards Institute.
    • Clinical evaluation of a urine transport kit with lyophilized preservative for culture, urinalysis and sediment microscopy.
      - Weinstein MP – Diag Microbiol Infect Dis. 1985;3:501-508
    • Quality assurance in urinalysis.
      - Haber MH. Clinics in Lab Med. 1988;8:432-436
    • Timeliness of urinalysis
      - Howanitz PJ, et al. A College of American Pathologists Q-Probes study of 346 small hospitals.Arch Pathol Lab Med. 1997;121:667-672
    • Is a preservative required for routine urinalysis?
      - Rafael J, et al. Am J Clin Pathol. 1997;108:344.
    • Evaluation of a standardized procedure for counting microscopic cells in body fluids
      - Emerson, JF and Emerson SS. J Clin Lab Anal 19:267-275 (2005)
    • Zell - und Partikelzählung im Urin: Vergleich von Durchfluss-Zytometrie UF-100, KOVA-Zählkammersystem und Urinsediment
      - Ottiger, C and Huber, AR. LabmedMay:172-176 (2003)
    • Standardization of urine particle counting in Switzerland: Flow cytometry UF-100 and disposable KOVA® Cell Chamber System
      - Ottiger, C and Huber, AR
    • Urinalysis: A comprehensive review
      - Somerville JA, Maxted WC and Pahira JJ. Am Fam Physician. 71(6):1153–1162, 2005
    • Quantitative urine particle analysis: integrative approach for the optimal combination of automation with UF-100 and microscopic review with KOVA® cell chamber
      - Ottiger C, Huber AR. – Clin Chem. 2003 Apr;49(4):617-23
    • Evaluation of the Sysmex UF-100 automated urinalysis analyzer
      - Ben-Ezra J, Bork L, McPherson RA. – Clin Chem. 1998;44 (1):92-5
    • Evaluation of a standardized procedure for counting microscopic cells in body fluids
      - Emerson JF, Emerson SS. J Clin Lab Anal. 19 (12): 267-275, 2005
    • Quantitative urine particle analysis: integrative approach for the optimal combination of automation with UF-100 and microscopic review with Kova cell chamber
      - Ottiger C, Huber AR. Clin Chem. 49 (4): 617-623, 2003
    • Zell- und PartikelzählungimUrin: Vergleich von Durchfluss-Zytometrie UF-100, KOVA-Zählkammersystem und Urinsediment (Article in German)
      - Ottiger C, Huber AR. Labmed. 2003: 172-176, 2003
    • Evaluation of the Sysmex UF-100 automated urinalysis analyzer
      - Ben-Ezra J et al. Clin Chem. 44 (1): 92-95, 1998
    • Comparison of various methods for the enumeration of blood cells in urine
      - McGinley M et al. J Clin Lab Anal. 6 (6): 359-361, 1992
    • Disposable plastic and reusable glass hemacytometers for cell counts
      - Bertholf MF, Kao KJ. Lab Med. 22 (12): 864-868, 1991
    • Quantitative urine microscopic examination using disposable counting chamber for diagnosis of urinary tract infection (Article in Japanese)
      - Hida Y et al. RinshoByori. 43 (12): 1273-1278, Dec 1995
    • Evaluation of yellow IIS/model 450 for routine urinalysis (Article in Chinese)
      - Huang LY et al. Chung Hua I HsuehTsaChih (Taipei). 53 (6 Suppl B): 23-30, Jun 1994
    • Reliability of pyuria detection method
      - Saito A, Kawada Y. Infection. 22 (Suppl): S36-37, 1994
    • On the performance and reliability of mechanized urine test strip measurement in comparison with visual reading
      - Marx AM et al. J ClinChemClinBiochem. 27 (7): 433-443, Jul 1989
    • Use of KOVA-Slide II with grid and uncentrifuged segmented urine specimens in the diagnosis of nongonococcal urethritis: a quantitative technique
      - Perera SA. Sex Transm Dis. 12 (1): 14-8, Jan-Mar 1985
    • Examination of urine sediment using the MD-KOVA system (Article in German)
      - Bauer HW. Med Welt. 33 (24): 875-878, Jun 18 1982
    • MD-KOVA system for analysis of urine sediments (Article in German)
      - Bauer HW. Med Lab (Stuttg). 35 (2): 31-35, 1982
    • Test strips for rationalising microscopic tests of urinary sediment (Article in German)
      - Kutter D. Dtsch Med Wochenschr. 105 (36): 1246-1249, Sep 5 1980
    • Quality control in professional practice in clinical chemistry: a companion text
      - Miller WG. ed DR Dufour.Washington, DC: AACC Press, 1999:12-1 to 12-22
    • Quality control of test systems waived by the clinical laboratory improvement amendments of 1988. Perceptions and practices
      - LaBeau KM, et al. Arch Pathol Lab Med. 2000;124:1122-1127
    • User Protocol for Evaluation of Qualitative Test Performance; Approved
    • Guideline—Second Edition
      - Clinical and Laboratory Standards Institute (CLSI). CLSI document EP12-A2 (ISBN 1-56238-654-9). Clinical and Laboratory Standards Institute
    • Commutability limitations influence quality control results with different reagent lots
      - Miller WG, Erek A, Cunningham TD, et al. – Clin Chem. 2011;57:76-83

    Product Documents

    KOVA® SDS and Value Sheets

    - Click here to obtain the latest value sheets for your product.


    Safety Data Sheets

    Controls & Stains
    Kova International, Inc.
    • 7272 Chapman Avenue, Suite B,
      Garden Grove, CA 92841

    • Phone: +1(714) 902-1700

    • Toll Free: +1 (855) 217-6399

    • Email: Kova International Support

    Biochemical Diagnostics, Inc.
    • 180 Heartland Blvd.
      Edgewood, NY 11717

    • Phone: +1 (631) 595-9200

    • Toll Free: +1 (800)-223-4835

    • Email: BCD Support